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Vector Biology & Gene Manipulation Mcqs

Q:

Embryonic stem cells are __________

A) Totipotent B) Pluripotent
C) Dead D) Multipotent
 
Answer & Explanation Answer: B) Pluripotent

Explanation: Embryonic stem cells can be cultured like any established cell line, but they are derived from the very early mouse embryo and are therefore pluripotent.

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Q:

Gene-knockout is the generation of a null-gene.

A) True B) False
 
Answer & Explanation Answer: A) True

Explanation: When the aim is to inactivate the target gene completely and generate a null allele, the term gene knockout is often used.

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52
Q:

Mutations can be introduced by ____________

A) Cloning B) Recombination
C) Ligation D) Gene targeting
 
Answer & Explanation Answer: D) Gene targeting

Explanation: Mutations can be introduced into predefined genes in vivo through a process termed as gene targeting which involves homologous recombination.

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Q:

Level of inhibition does not depend on ________ of antisense RNA.

A) Size B) Temperature
C) Ph D) Range
 
Answer & Explanation Answer: A) Size

Explanation: The level of inhibition apparently does not depend on the size of the antisense RNA or the part of the endogenous gene to which it is complimentary.

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43
Q:

Which gene is targeted in transgenic tomato plant?

A) Mg B) Pg
C) Gfp D) Mbp
 
Answer & Explanation Answer: B) Pg

Explanation: Smith in 1988 generated transgenic tomato plants carrying an antisense construct targeting the endogenous polygalacturonase gene.

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50
Q:

What is MBP?

A) Inducer B) Protein
C) Promoter D) Gene
 
Answer & Explanation Answer: B) Protein

Explanation: An expression cassette was constructed in which the mouse myelin basic protein CDNA was inverted with respect to the promoter.

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33
Q:

Which type of inhibition can be achieved using antisense RNA?

A) Stable B) Unstable
C) Transient D) Integrative
 
Answer & Explanation Answer: C) Transient

Explanation: Transient inhibition of particular genes can be achieved by directly introducing antisense RNA or antisense oligonucleotides into cells.

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34
Q:

Antisense RNA can be used in eukaryotes.

A) True B) False
 
Answer & Explanation Answer: B) False

Explanation: Antisense RNA is used as a natural mechanism to regulate gene expression in a number of prokaryote systems and to a lesser extent in other organisms.

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