Molecular Biology Mcqs

Q:

What is the source of energy during the action of SPO11?

A) Cleavage of peptide bond B) Cleavage of amide bond
C) Cleavage of glycoside bond D) Cleavage of phosphodiester bond
 
Answer & Explanation Answer: D) Cleavage of phosphodiester bond

Explanation: During the action of SPO11 the cleavage of DNA phosphodiester bond is stored in the bound protein-DNA linkage. By this process, the DNA strands can be released.

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Q:

What is the cleavage site of RuvC?

A) 5’….A/TTTG/C….3’ B) 5’….G/TTTC/T….3’
C) 5’….T/TGCG/C….3’ D) 5’….C/ATTG/T….3’
 
Answer & Explanation Answer: A) 5’….A/TTTG/C….3’

Explanation: Cleavage by RuvC takes place at sites conforming to the consensus sequence 5’….A/TTTG/C….3’. cleavage occurs after the second T in this sequence.

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35
Q:

After cleaved by RuvC the resulting ligated recombination products will only be the splice product.

A) True B) False
 
Answer & Explanation Answer: B) False

Explanation: Depending on which pair of strands is cleaved by RuvC the resulting recombinant product will depend. It either forms a spliced product or a patched product.

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31
Q:

Which component cleaves the specific DNA strands at the Holliday junction?

A) RuvC B) RuvD
C) RuvA D) RuvB
 
Answer & Explanation Answer: A) RuvC

Explanation: RuvC recognizes the Holliday junction. It specifically nicks two of the homologous DNA strands that have the same polarity.

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Q:

The RuvAB complex specifically recognizes the Holliday junction in an ATP dependent manner.

A) True B) False
 
Answer & Explanation Answer: A) True

Explanation: RuvA recognizes and binds to the Holliday junction and recruits RuvB protein to this site. RuvB is a hexameric ATPase thus making the process an ATP dependent one.

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37
Q:

What is the joint molecule?

A) RecB of the RecBCD pathway B) B DNA
C) Z DNA D) Rec A bound three stranded structure
 
Answer & Explanation Answer: D) Rec A bound three stranded structure

Explanation: The RecA bound three stranded structure is known as the joint molecule. It usually contains several hundred base pairs of hybrid DNA.

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33
Q:

What is the central protein of homologous recombination?

A) RecB B) RecD
C) RecA D) RecC
 
Answer & Explanation Answer: C) RecA

Explanation: RecA is the central protein of homologous recombination. It is the founding member of a family of enzymes called the strand exchange protein.

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30
Q:

If the foreign DNA lacks the Chi site what will happen?

A) Chi site of DNA will destroy B) Foreign DNA is not affected
C) Foreign DNA easily enters the cell D) DNA sequence will be intact as previous
 
Answer & Explanation Answer: C) Foreign DNA easily enters the cell

Explanation: RecBCD sometimes function simply to destroy DNA as it does when foreign DNA lacking frequent Chi sites enters cells. RecBCD can protect cells from the potentially deleterious consequences of taking up foreign sequences.

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