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Microbiology Mcqs

Q:

Electron Microscope can give a magnification up to ___________

A) 400,000X B) 100,000X
C) 15000X D) 100X
 
Answer & Explanation Answer: A) 400,000X

Explanation: The resolving power of the electron microscope is more than 100 times that of the light microscope, and it produces useful magnification up to 400,000X. It is possible to resolve objects as small as 10 Angstrom.

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738
Q:

Isolation of pure culture refers to ___________

A) purification of culture B) introduction of inoculum
C) separation of a single colony D) to grow microorganisms on a surface
 
Answer & Explanation Answer: C) separation of a single colony

Explanation: To study the characteristics of one species, that species must be separated from all the other colonies of species, i.e., it must be isolated in pure culture.

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549
Q:

Zone of inhibition is observed in tube-dilution method.

A) True B) False
 
Answer & Explanation Answer: B) False

Explanation: Small paper disks impregnated with known amounts of chemotherapeutic agents are placed upon the surface of an inoculated plate. So after incubation in disk-plate methods, a zone of inhibition around the disk indicates that the organism was inhibited by the drug.

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505
Q:

Thiobacillus can produce an acidity in the range of pH __________

A) 7 B) 5
C) 3 D) 1
 
Answer & Explanation Answer: D) 1

Explanation: Organisms of the genus Thiobacillus oxidize elemental sulphur to sulphuric acid and can produce acidity in the range of pH 1.

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392
Q:

Which of the following genus of bacteria do not have a fermentative type of metabolism?

A) Bacteroides B) Fusobacterium
C) Wolinella D) Selenomonas
 
Answer & Explanation Answer: C) Wolinella

Explanation: Wolinella do not have a fermentative type of metabolism. They respire anaerobically with hydrogen or formate as electron donors and fumarate or nitrate as electron acceptors.

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378
Q:

The greatest resolution in light microscopy can be obtained with ___________

A) Longest wavelength of visible light used B) An objective with minimum numerical aperture
C) Shortest wavelength of visible light used D) Shortest wavelength of visible light used and an objective with the maximum numerical aperture
 
Answer & Explanation Answer: D) Shortest wavelength of visible light used and an objective with the maximum numerical aperture

Explanation: The relationship between numerical aperture (NA) and resolution is:-
Resolution (d) = wavelength / 2(NA)
Thus maximum resolution is obtained with the shortest wavelength of visible light and an objective with the maximum NA.

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339
Q:

Total Magnification is obtained by __________

A) Magnifying power of the objective lens B) Magnifying power of eyepiece
C) Magnifying power of condenser lens D) Magnifying power of both the objective lens and eyepiece
 
Answer & Explanation Answer: D) Magnifying power of both the objective lens and eyepiece

Explanation: The total magnification is determined by multiplying the magnifying power of the objective by that of the eyepiece. Generally, an eyepiece having a magnification of 10X is used although eyepieces of higher or lower magnifications are available.

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307
Q:

Oil immersion objective lens has an NA value of____________

A) 0.65 B) 0.85
C) 1.33 D) 1.00
 
Answer & Explanation Answer: C) 1.33

Explanation: NA = refractive index * sine (half-aperture angle).
The maximum NA for a dry objective is less than 1.0 as the refractive index of air is 1. The values of NA for oil immersion lens is slightly greater than 1.0 in the range of (1.2 to 1.4) as the refractive index of oil is 1.56.

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