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Mcqs

Genetic Engineering Mcqs

Our 1000+ Genetic Engineering MCQs (Multiple Choice Questions and Answers) focus on all areas of Genetic Engineering covering 100+ topics. These topics are chosen from a collection of the most authoritative and best reference books on Genetic Engineering. One should spend 1 hour daily practicing these MCQs for 2-3 months to learn and assimilate the Genetic Engineering subject comprehensively. This way of systematic learning will prepare anyone easily for Genetic Engineering exams, contests, online tests, quizzes, MCQ-tests, viva-voce, interviews, and certifications.

 

Genetic Engineering Multiple Choice Questions Highlights

  • 1000+ Multiple Choice Questions & Answers (MCQs) in Genetic Engineering with a detailed explanation of every question.
  • These MCQs cover theoretical concepts, true-false(T/F) statements, fill-in-the-blanks, and match the following style statements.
  • These MCQs also cover numerical as well as diagram-oriented MCQs.
  • These MCQs are organized chapterwise and each Chapter is further organized topicwise.
  • Every MCQ set focuses on a specific topic of a given Chapter in the Genetic Engineering Subject.

 

Who should Practice Genetic Engineering MCQs?

  • Students who are preparing for college tests and exams such as mid-term tests and semester tests on Genetic Engineering.
  • Students who are preparing for Online/Offline Tests/Contests in Genetic Engineering.
  • Students who wish to sharpen their knowledge of the Genetic Engineering Subject.
  • Anyone preparing for an Aptitude test in Genetic Engineering.
  • Anyone preparing for interviews (campus/off-campus interviews, walk-in interviews and company interviews).
  • Anyone preparing for entrance examinations and other competitive examinations.
  • All - Experienced, Freshers and College / School Students.

 

Genetic Engineering Chapters

Here's the list of chapters on the "Genetic Engineering" subject covering 100+ topics.

  • Basics of Genetic Engineering
  • Polymerase Chain Reaction
  • Simple Cloning
  • Other Vector Systems for E. Coli
  • Libraries Construction
  • Screening Libraries
  • Modifications and Mutagenesis
  • Use of Cloned DNA
  • Using Other Organisms
  • Genetic Engineering

 

Latest Popular
Q:

The term ‘endonuclease’ refers to cutting the DNA sequence from ______________

A) only within the polynucleotide chain, not at the ends B) the ends of the chain
C) anywhere in the chain D) exactly in the middle of the chain
 
Answer & Explanation Answer: A) only within the polynucleotide chain, not at the ends

Explanation: The cleavage is done within the polynucleotide chain and not at the ends. The enzyme which cuts the sequence at the ends is known as exonuclease.

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Category: Biotech Engineering MCQs
Sub Category: Genetic Engineering Mcqs

317
Q:

Choose the incorrect statement for the method homopolymer tailing.

A) The first step is the RNA: DNA hybrid synthesis B) Terminal transferase is used for the addition of nucleotides on 3’ end
C) Terminal transferase adds only at DNA strands D) The DNA strand is now having known sequence at 3’ end
 
Answer & Explanation Answer: C) Terminal transferase adds only at DNA strands

Explanation: Homolpoymer tailing is also used for cDNA synthesis. The first step remains same as the other methods, which is the synthesis of RNA: DNA hybrid. It is then treated by terminal transferase, the enzyme is responsible for adding nucleotides on 3’ end of both DNA and RNA. The 3’ end is now having a known DNA sequence and it is used as a tail in the reaction.

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Category: Biotech Engineering MCQs
Sub Category: Genetic Engineering Mcqs

246
Q:

Primers are generally ____________

A) 20-30 nucleotides long B) 40-50 nucleotides long
C) as long as the template is D) taken according to the amount available
 
Answer & Explanation Answer: A) 20-30 nucleotides long

Explanation: Primers are generally short in length. They are 20-30 nucleotides long. It is easier to match short primers with the template in comparison to long primers. But in the case of eukaryotic DNA as a template, long primers are preferred.

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Category: Biotech Engineering MCQs
Sub Category: Genetic Engineering Mcqs

230
Q:

Which of the following is favoured for primer design?

A) The melting temperature should be different for both the primers B) Primers should be long in length
C) Primers should not be complementary to each other D) Matching should be of whole primer to the template
 
Answer & Explanation Answer: C) Primers should not be complementary to each other

Explanation: Primers should not be complementary to each other. It is so because if they are complementary, primer dimer formation takes place. If primer dimer is formed, proper elongation won’t be taking place.

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Category: Biotech Engineering MCQs
Sub Category: Genetic Engineering Mcqs

229
Q:

Phage based recombination systems are used for ____________

A) cleaving the molecules at specific sites B) adding the molecules at specific sites
C) breakage and rejoining the molecules at specific sites D) breakage at random sites
 
Answer & Explanation Answer: C) breakage and rejoining the molecules at specific sites

Explanation: Phage based recombination systems are used for breaking and rejoining the molecules at specific sites and not at random sites.

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Category: Biotech Engineering MCQs
Sub Category: Genetic Engineering Mcqs

191
Q:

What will be the consequence of not having an origin of replication (ori) in the vector?

A) If an ori is absent, replication of vector would not take place B) As the cells divide after taking up the vector, both the daughter cells would be having the vector
C) A colony of transformed colonies is observed D) The vector won’t be taken up by the cell
 
Answer & Explanation Answer: A) If an ori is absent, replication of vector would not take place

Explanation: In the case ori is absent, the vector won’t be able to replicate. As the replication won’t take place, only one of the daughter cells would be having the vector. A colony of transformed colonies won’t be obtained. The puc18 ori came from a plasmid in clinical bacterial isolate and was called as pMB1.

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Category: Biotech Engineering MCQs
Sub Category: Genetic Engineering Mcqs

189
Q:

Isoschizomers are defined as ___________

A) enzymes having same recognition sequence and always cutting at the same site B) enzymes having same recognition sequence and always cutting at different site
C) enzymes having different recognition site and cutting at the same site D) enzymes having same recognition site and they may or may not cut at the same site
 
Answer & Explanation Answer: D) enzymes having same recognition site and they may or may not cut at the same site

Explanation: Isoschizomers are the enzymes which are having same recognition sequence but they necessarily don’t cut at the same site. DraI and AhaIII both recognize and cleave at TTT|AAA, whereas on the other hand, ApaI cuts at GGGCC|C and Bspl20I cuts at G|GGCCC.

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Category: Biotech Engineering MCQs
Sub Category: Genetic Engineering Mcqs

150
Q:

Choose the incorrect statement with respect to rolling circle replication.

A) It is for a single replication fork and yields a concatemeric molecule B) Staggered cleavage of DNA takes place at the cos sites
C) The cleavage generates 10 nucleotide long overhangs D) Without the cos sites, the packaging won’t be possible
 
Answer & Explanation Answer: C) The cleavage generates 10 nucleotide long overhangs

Explanation: The second phase constitutes of rolling circle mode. It is done with a single replication fork and yields concatemeric molecules. They are required for assembly into mature phage particles. The packaging region should be flanked by the cos sites. Phage DNA is inserted into the phage head and the cos sites are brought adjacent to each other. It is followed by cleavage at cos sites and it leaves 12 nucleotide long staggered ends. The cos sites are required for packaging to take place.

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Category: Biotech Engineering MCQs
Sub Category: Genetic Engineering Mcqs

134