Q:
If a putative protein sequence is cloned in an expression vector and the expressed protein is not showing protease activity, then which of the following is not correct?
Answer & Explanation
Answer: A) The protein is not protease
Explanation: If the putative protein sequence is cloned and the expressed protein is not showing protease activity, it is not necessary that it is not protease. Suppression of protease activity can be because of incorrect folding or that some cofactor is required for protease activity. The most commonly used expression system is E.coli.
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