Q:
Which of the following can be used to clone DNA sequence of size larger than 25 kb?
Answer & Explanation
Answer: A) Yeast artificial chromosome
Explanation: For cloning DNA sequences of a size larger than 20 to 25 kb, the conventional vectors such as plasmids and bacteriophages can not be used. For the cloning of large DNA fragments, the yeast artificial chromosome (YAC) may be used.
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